ChromaLink™ One-Shot Antibody Biotinylation Kit


Single-reaction labeling kit with biotinylation reproducibility
The ChromaLink™ One-Shot Antibody Biotinylation Kit provides convenient, measurable biotinylation of 100 µg of antibody. The kit contains ChromaLink™ Biotin, which incorporates a UV-traceable chromophore, based on Solulink’s proprietary linking technology, to enable reproducibility in your biotinylation process. Now you can measure the degree of biotinylation in minutes, not hours, without the standard curves required for HABA/avidin and fluoro-reporter assays. With a simple and direct UV scan (A280 and A354), you can quantify biotin incorporation and ensure reproducible production of consistent batches.

  Download White Paper: How to Biotinylate with Quantifiable Results

The ChromaLink™ One-Shot Antibody Biotinylation Kit includes everything you need to biotinylate your antibody:  buffers, reagents, spin columns, and easy-to-follow protocols. With the ChromaLink™ One-Shot Antibody Biotinylation Kit, you can biotinylate and purify 100 µg of antibody in about 2.5 hours. The resulting biotinylated antibody is ready for binding to streptavidin. We recommend our NanoLink™ Streptavidin Magnetic Beads (Cat. No. M-1002-010) with the highest biotin-binding capacity available for high-sensitivity ELISA, western blot, IHC, or immunoassay development.

ChromaLink™ One-Shot Antibody Biotinylation Kit Advantages

  • Reproducible Production UV-traceable chromophore permits nondestructive, rapid A280/A354 quantification of incorporated biotin, enabling consistency and reproducibility
  • Extended PEG3 Spacer – Helps reduce aggregation, minimizes steric hindrance, and enhances solubility
  • One-shot – All components sized to quickly label 100 µg of antibody
  • Convenient – Kit contains buffers, spin columns, and calculator to determine MSR

Solulink ChromaLink One-Shot Antibody Biotinylation Kit
Pierce EZ-Link Sulfo-NHS-
Biotinylation Kit
Life Technologies APEX
Biotin-XX Antibody Labeling Kit
Biotin Quantification
Yes
No
No
Complete Kit
Yes
No
No
Purification
Yes
No
No

Citations
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  2. Sharma RK, Schabowsky RH, Srivastava AK, Elpek KG, Madireddi S, Zhao H, Zhong Z, Miller RW, MacLeod KJ, Yolcu ES, Shirwan H. 4-1BB Ligand as an Effective Multifunctional Immunomodulator and Antigen Delivery Vehicle for the Development of Therapeutic Cancer Vaccines. Cancer research 2010;70(10):3945.
  3. Dirksen A, Yegneswaran S, and Dawson PE. Bisaryl Hydrazones as Exchangeable Biocompatible Linkers.Angew. Chem. Int. Ed. 2010,49,2023–2027.
  4. Srinivasan B, Li Y, Jing Y, et al. A detection system based on giant magnetoresistive sensors and high-moment magnetic nanoparticles demonstrates zeptomole sensitivity: potential for personalized medicine.Angew. Chem. Int. Ed. Engl. 2009;48(15):2764-2767.
  5. Ma Z, Li Q, An H, Pankonin MS, Wang J, Loeb JA. Targeting Human Epidermal Growth Factor Receptor Signaling with the Neuregulin's Heparin-binding Domain. Journal of Biological Chemistry 2009;284(46):32108.