TARGATT™ Transgenic Kit is designed to create site-specific, knock-in transgenic mice in a more efficient and significantly faster way over traditional methods. Generating transgenic mice by conventional methods (e.g. pronuclear microinjection or lentiviral injection) has several limitations, first of which is random insertion of the transgene. Random insertion of a transgene results in a position effect where either the transgene is prone to silencing or endogenous gene expression is disrupted. Furthermore, this position effect is compounded when a transgene is inserted as multiple copies, resulting in instability at the insertion locus.
Using our proprietary site-specific DNA integration system, TARGATT™ Transgenic Kit, combined with our genetically TARGATT™ mouse embryos (cat.# AST-0001, AST-0002, AST-0003, AST-0004). Applied StemCell Inc. (ASC) offers high quality H11(Hipp11) and ROSA26 transgenic mouse kits with our TARGATT™ technology. Using our TARGATT™ Technology, you can generate your own knock-in mouse in just three months.
Fast & Site-Specific Knock-in Mouse Technology
If you prefer to generate a knock-in mouse model on your own, TARGATT™ Embryo H11(Hipp11) or Rosa26 and Transgenic Kits (2 or 5 microinjection size) are available for purchase.
Applied Stem Cell Inc.’s proprietary TARGATT™ technology enables highly efficient site-specific gene integration in mammalian cells and animals1, 2. This technology uses øC31 integrase to insert any gene of interest into a docking site, pre-engineered in an intergenic region and transcriptionally active genomic locus. Our TARGATT™ technology improves several aspects in the generation of transgenic cell lines and animals: (1) High integration efficiency mediated by øC31 integrase reduces time and cost; (2) Site-specific integration at a pre-selected genomic locus eliminates position effect and ensures high level expression of the transgene; (3) Integration at intergenic region ensures that no internal genes are interrupted; (4) Single copy gene integration eliminates repeat-induced gene silencing and genomic instability; (5) Site-specific integration allows a precise comparison of the effects of the transgenes among different lines. TARGATT™ technology can be utilized for a variety of applications including reporter gene expression, gene knockdown, disease cell and animal models.