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shRNA-mir-clones


Advances in shRNA-mir design for potent and specific knockdown

PLATINUM Select microRNA-adapted short hairpin RNA (shRNA-mir) are next generation vector-based RNAi triggers generated using the proprietary shERWOOD  algorithm  developed in Dr. Gregory Hannon’s laboratory at Cold Spring Harbor Laboratory. Based on the functional testing of  270,000 shRNA sequences using a high-throughput sensor assay (Knott et al2014) the shERWOOD algorithm has been trained to select the rare shRNA-mir designs that are consistently potent even at single copy representation in the genome. The new shERWOOD algorithm predicted sequences have been applied to the creation of new PLATINUM Select shRNA-mir collections targeting human and mouse genomes.

  •  shERWOOD algorithm designs ensure more efficient shRNA-mir processing and potency at single copy
  • microRNA-30 context increases knockdown specificity and reduces toxicity
  • All shRNA-mir designs are scored and ranked per gene
  • 100% Guaranteed* Knockdown

 

Hairpin and Vector Details


shRNA-mir Design
shRNA sequences consists of a 22 nt target-gene-specific sequence, a 19 nt loop, and another 22 nt reverse complementary sequence. shRNA sequences are embedded in a primary microRNA-30 context so as to undergo processing via the endogenous microRNA pathway. shERWOOD Algorithm based shRNA designs have been created for each target gene according to the following criteria:
  • Hairpin sequences target the coding regions of Refseq annotated genes
  • Multiple transcript genes were targeted by designs that spanned across all transcripts. In the few cases (less than 0.5%) where there was insufficient sequence design space that spanned across all transcripts - each transcript is designed against separately.
pZIP (SFFV) lentiviral vector 
pZIP Vector Schematic (detailed vector map)
pZIP Lentiviral shRNA vector

  • Deliver to a wide range of cell types including primary and non-dividing
  • Hybrid LTR for efficient packaging with 2nd, 3rd and 4th generation packaging systems
  • shRNA and fluorescent marker are expressed from a single transcript allowing direct visual detection of shRNA exrpresssion
  • Easily create stable cell lines withefficient integration and puromycin selection

pMLP retroviral vector
pMLP Vector Schematic (detailed vector map)
  • MSCV-based retroviral vector for delivery and expression in most mammalian cell lines including murine or human hematopoietic and embryonic stem (ES) cells.
  • shRNA-mir constructs are expressed from the retroviral LTR promoter.
  • Fluorescent marker for tracking transduced cells

 

 

Cat# Product Name
More information
TLHK9600 PLATINUM Select Human ZIP Lentiviral shRNA-mir - Starter kit
TLHS9600 PLATINUM Select Human ZIP Lentiviral shRNA-mir target gene set
TLMK9600 PLATINUM Select Mouse ZIP Lentiviral shRNA-mir - Starter kit
TLMS9600 PLATINUM Select Mouse ZIP Lentiviral shRNA-mir target gene set
TRHS1000 PLATINUM Select Human MLP Retroviral shRNA-mir - Target gene set
TRHK1000 PLATINUM Select Human MLP Retroviral shRNA-mir - Starter kit
TRH1000 PLATINUM Select Human MLP Retroviral shRNA-mir - Individual construct
TRMS1001 PLATINUM Select Mouse MLP Retroviral shRNA-mir - Target gene set
TRMK1001 PLATINUM Select Mouse MLP Retroviral shRNA-mir - Starter kit
TRM1001 PLATINUM Select Mouse MLP Retroviral shRNA-mir - Individual construct
TRH3500 PLATINUM Select Human MLP Retroviral shRNA-mir genome library
TRM3500 PLATINUM Select Mouse MLP Retroviral shRNA-mir genome library