Electro Elution method with Gentaurflex-tube
Gentaur has developped a new tool which provide highly efficient molecule extraction from any gel. Gentaurflex-tube process is organized to accomodate small or large gel slice inside the Gentaurflex-tube. Gentaurflex-tub extract any molecule ranging from 3,5kDa to unlimited size molecule. Gentaurflex-tube chamber together with the gel slice, is buffer filled.
Using electro elution Gentaurflex-tube supporting tray, position each tube membrane surface perpendicular to current flowing from the electrodes, so as to provide membrane maximum surface exposure to electrical flow of current.
HIGH TROUGH-PUT PURIFICATION
Protein preparation for MALDI-TOF-MS
Sodium dodecylsulfate-polyacrylamide gel electrophoresis (SDS-PAGE) is a widely used technique for the separation and estimation of the molecular weight of individual proteins.
The accuracy of molecular weight determination & characterization is limited by protein low recovery. Recently, MALDI-TOF-MS has found widespread use for the determination of molecular mass of intact proteins isolated from gels. Isolation of proteins from gels with the newly developed Gentaurflex-tube electro-elution system delivers over 80% recovery yields. A combination of SDS-PAGE, Gentaurflex-tube electro-elution system, and MS buffer removing SDS (included in kit), delivers MALDI-TOF-MS, and greater accuracy in the determination of protein molecules. Now we can analyze low yield and complex proteins, such as integral membrane proteins (hydrophobic) or high molecular masses protein.
Gentaurflex-tube Protein gel extraction
Electro elution with Gentaurflex-tube provides rapid, secure, efficient tools extracting protein from polyacrylamide gel. Minimum guarenteed protein recovery is above 70%.
Oligo PAGE purification
30 min Oligo PAGE purification. Gentaur has developed a new short protocol for Oligo PAGE purification. The present method for oligo PAGE purification is a lengthy process (overnight) and provides a low yield, (the diffusion method provides a low 20-30% recovery).
DNA, RNA and large DNA gel extraction
High throughput electro elution with Gentaurflex-tube using standard electrophoresis unit
Picture shows 24 Midi Gentaurflex-tube electro eluting, typical 50kDa/ 80min
This gel extraction kit extracts small nucleic acid fragments such as DNA Oligonucleotides (down to 15nt) and RNA molecules. It can also be used to extract protein-nucleic acid complexes and large size DNA fragments (>100kb) from agarose or polyacrylamide gel. Gentaurflex-tube is based on a single-use tube.