Protein staining solutions


Protein Silver Staining GSS050

GentaurSilver Staining for proteins  in polyacrylamide gels  

  •         Kit solutions are sufficient for 20 mini gels
  •         Kit protocol adjusted for 1.5 or 1.0 mm thick gel
  •         Microwave oven fast silver staining protocol
  •         Staining detect as low as 0.1 ng BSA
  •         A protocol which results no Background
  •         Protocol free from dark spots
  •         even staining or contaminant bands
  •         Unique destaining protocol and buffers
  •         Unique buffers and protocol for preparation of proteon sample for MALDI-TOF-MS analysis
  •         Sensitizing solution DOES NOT contain glutaraldehyde

 

Staining of Protein for Gel extract SB010

Description:

SeeBand protein staining solution guarantees protein staining in polyacrylamide gel without fixation of the protein to the gel. This unique staining solution allows the electro-elution of a desired intact protein from polyacrylamide gels with high recovery yield. The staining solution provides tracking staining intensity during the course of the staining process with low background. No need for multi-step washing as required with other gel staining procedures. Washing utilizes water only, further enhancing staining sensitivity while providing a clear, high contrast background.

Application:

  • Staining of protein in polyacrylamide gels specific for high yield recovery of proteins.
  • Stainig of proteins in gels before Western Blot.
  • Protein staining without inactivating the protein.
  • Staining of 2D gel.

Specifications:

  • Volume: 500 ml.
  • Number of gels: 25 mini ( 10 x 10 cm ).
  • Sensitivity : 20 ng protein per band.
  • Low background. No need for multi step washing.
  • Washing uses water only.
  • No denatured material affecting the protein during the staining

Kit Content:

  • 500 ml protein staining solution
  • Handbook

 


 

Staining of Protein for Gel extract SB010

 

Description:

SeeBand protein staining solution guarantees protein staining in polyacrylamide gel without fixation of the protein to the gel. This unique staining solution allows the electro-elution of a desired intact protein from polyacrylamide gels with high recovery yield. The staining solution provides tracking staining intensity during the course of the staining process with low background. No need for multi-step washing as required with other gel staining procedures. Washing utilizes water only, further enhancing staining sensitivity while providing a clear, high contrast background.

Application:

  • Staining of protein in polyacrylamide gels specific for high yield recovery of proteins.
  • Stainig of proteins in gels before Western Blot.
  • Protein staining without inactivating the protein.
  • Staining of 2D gel.

Specifications:

  • Volume: 500 ml.
  • Number of gels: 25 mini ( 10 x 10 cm ).
  • Sensitivity : 20 ng protein per band.
  • Low background. No need for multi step washing.
  • Washing uses water only.
  • No denatured material affecting the protein during the staining

Kit Content:

  • 500 ml protein staining solution
  • Handbook

 


See Band Forte Protein Staining Solution

Description:

 See Band Forte (catalog # SB020) a protein staining solution guarantees protein staining in polyacrylamide gels with fixation of the protein to the gel. This unique staining solution allows formation of sharp protein bands in the gel. The staining solution allows the visual tracking of the staining intensity during the course of the staining process with very low background. No need for multi-step washing as is required with other gel staining procedures. Washing utilizes water only, which further enhances staining sensitivity and provides a clear, contrasted background.

Specifications:

  • Volume: 500 ml.
  • Number of gels: 25 mini ( 10 x 10 cm ).
  • Sensitivity: 10 ng protein per band.
  • Low background. No need for multi step washing
  • Washing utilizes water only.

Kit Content:

  • 500 ml protein staining solution
  • Handbook

 

Membrane Staining solution SB040

Description:

The need to check the transfered Protein, and the quality of transfer onto membrane before Western blot, is a basic requirement for any experienced molecular scientist. Removing the staining, once transfer has been visualized, typically takes only 5 min.

Avantages:

  • Secures the transfer to the membrane
  • High sensitivity detection 50 ng protein / band (BSA).
  • Rapid staining protocol , time required is only 1 to 10 minutes.
  • Membrane staining  with SB040 is economical, the staining medium can be reused up to 10 times.
  • Simple & rapid de-staining, within just a few minutes.
  • Destaining of the membrane is complete, no interference with immunoblotting.
  • Ideal companion to Western blot

 

Gentaur Silver Stain