Stripping buffer

Antibody Stripping Buffer


Western Blots are widely used to detect and compare proteins from a complex mixture utilizing antibody detection on a membrane, (membrane based antibody detection). Chemiluminescence has become an easy and sensitive method of detection compared to other analysis methods.  The nature of chemiluminescence detection enables the reprobing of the separated protein mixture on the membrane. Conventionally, Western blots have been stripped under extremely harsh conditions that may alter the antigen for subsequent immunoprobing. Gene Bio-Application Stripping buffer is a unique formula that provides a gentle method of removing primary and secondary antibodies from the membranes thereby enabling several reprobings of the same membrane